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filingDate 2011-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_386966c6f53a07997e97da5772d2bf88
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publicationDate 2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-PI1105460-A2
titleOfInvention FAST IDENTIFICATION METHOD OF OUTBREAKING MICOBACTERY CEPA AND DIAGNOSTIC KIT
abstract FAST IDENTIFICATION METHOD OF OUTBREAKING MICOBACTERY CEPA AND DIAGNOSTIC KIT. The present invention is directed to a method of rapidly identifying the outbreak-causing mycobacterium strain in surgeries by performing multiplex PCR steps of three regions of the mycobacterial genome using primers (SEQ. ID. NO. 1 to SEQ. ID 6) and reverse hybridization of the amplified fragments with specific oligonucleotides (SEQ. ID. NO. 7 to SEQ. ID. NO. 12) fixed on transfer membranes for application to nucleic acid and proteins. Additionally, the present application provides a diagnostic kit for carrying out the method of the present invention wherein the identification of the outbreak strain is based on a single base polymorphism (SNP) observed in the rpoB gene sequence.
priorityDate 2011-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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