http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-PI1016151-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b1c7560aa50b0da1769cd5b6f735b69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca45d719b6774f7cbaf1ddd7ac03a5de |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1072 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K7-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate | 2010-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70c8e79f8ef6a04843db808d36a65bbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e147075bdb51ada610e3866e3d5a4fe2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1a3ecb5634a4d11446c37ed71a7704e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0e29b75cbd24ce06c33f4684a771de4 |
| publicationDate | 2021-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | BR-PI1016151-B1 |
| titleOfInvention | process for producing a peptide thioester, processes for producing a polypeptide, process for producing a second intermediate used for the process for producing the peptide thioester and process for removing a tag for purification added to a c-terminal side of a recombinant protein |
| abstract | process for production of peptide thioester. a new process is provided for the chemical conversion of a peptide chain to a peptide thioester. the inventors paid attention to the cysteine residue of a peptide chain. as a result, the following was found. when a -c (= x) -r1 group is introduced into the thiol group of the cysteine residue and then the resulting peptide is reacted with a compound having a leaving group, represented by the formula: -nh-c (= y) nhr3 in an organic solvent, the -nh-c (= y) nhr3 group bonds through the addition reaction to the carboxyl group of the n-terminal side peptide bond of the cysteine residue, through which the peptide bond is cleaved and the lateral c-terminal peptide fragment is cut. moreover, when the resulting peptide chain having the -nh-c (= y) nhr3 group is reacted with a thiol in a buffer solution, a thiol exchange reaction occurs, namely, the thiol group of the thiol binds to the carbonyl carbon to which the -nh-c (= y) nhr3 group is attached, through which the -nh-c (= y) nhr3 group is eliminated. thus, conversion to a peptide thioester is achieved. |
| priorityDate | 2009-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 137.