http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-PI1013548-B8

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filingDate 2010-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_021e648f4b05d8eb57ece1c66b6ac36b
publicationDate 2021-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-PI1013548-B8
titleOfInvention industrial purification method of biologically active phycotoxins
abstract industrial purification method of biologically active phycotoxins. this invention relates to a method of industrial purification of biologically active phycotoxins which allows to provide an adequate amount of phycotoxins; if the source of phycotoxins is a pellet, it is lysed using appropriate methods; the material from cyanobacterial lysis is subjected to cold extraction; obtaining a concentrate from the aqueous phase obtained in the previous step or from the culture medium from the first step; centrifuge the concentrate to obtain a supernatant; pass the supernatant through a column of diatomaceous earth and obtain an eluate of the phycotoxin; the eluate obtained in the previous step is passed through activated carbon columns; the eluate from the previous step is again passed through a column of diatomaceous earth; the eluate from the previous step is placed in the aqueous phase; the partially purified extract from the previous step is subjected to hplc prepared in several steps, obtaining pure biologically active phycotoxin.
priorityDate 2009-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

Incoming Links

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Total number of triples: 25.