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filingDate 2010-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_021e648f4b05d8eb57ece1c66b6ac36b
publicationDate 2020-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-PI1013548-B1
titleOfInvention industrial purification method of biologically active phytotoxins
abstract method of industrial purification of biologically active phytotoxins this invention relates to a method of industrial purification of biologically active phytotoxins that allows to provide an adequate amount of phytotoxins; if the source of phytotoxins is a pellet, it is subjected to lysis using appropriate methods; the material from cyanobacterial lysis is subjected to cold extraction; obtaining a concentrate from the aqueous phase obtained in the previous step or from the culture medium of the first step; centrifuge the concentrate to obtain a supernatant; pass the supernatant through a column of diatomaceous earth and obtain a phytotoxin eluate; the eluate obtained in the previous step is passed through activated carbon columns; the eluate from the previous step is again passed through a column of diatomaceous earth; the eluate from the previous step is placed in an aqueous phase; the partially purified extract from the previous step is subjected to hplc prepared in several steps, obtaining the pure biologically active phytotoxin.
priorityDate 2009-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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