http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-PI1001911-A8

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-16
filingDate 2010-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e98f84666f6f9702d975e9233a8e9e0
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publicationDate 2016-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-PI1001911-A8
titleOfInvention Process for platelet-rich plasma and growth factors for use in orthopedic surgery
abstract process for obtaining platelet-rich plasma and growth factors for use in orthopedic surgery. The procedure consists in the collection of 60 ml of the patient's whole blood, through the access routes, central venous or jugular, the accesses should be free of serum or any medications. This blood should be placed in glass tubes containing sodium citrate anticoagulant These tubes should then be manually centrifuged for 5 min at 2200 rpm, promoting the separation of red cells that, due to their higher molecular weight, deposit in the lower section of the tube, while in the upper section, the total plasma ( prp + ppp) with the platelets, and in the central section between the red cells and the total plasma, there is a thin whitish haze, which contains white cells, mainly leukocytes, and the larger platelets, with One 20 ml syringe is collected 2 ml of ppp from the total plasma from each tube, then into a dry tube, 1 ml of 10% calcium glucanate is placed along with 1 ml of ppp + 1 ml of ppp, obtaining autologous thrombin, this dry tube is deposited in a container with water heated to a temperature between 39-40 degrees, where it remains in a water-bath conducted for approximately 10 minutes. During this procedure, the remainder of the plasma (prp) and the mist zone (leukocytes) are aspirated and packed in 10 ml syringes, leaving the remaining plasma in the dry tube, ie a platelet-rich plasma (prp). ).
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