http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-PI0713795-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_adb0e564374fedf34b36103d36e6455a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-242 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate | 2007-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a7e07cc2f8bd4df92893c944966960f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c21d3e42c0db85655c7c5c467a11a5f |
| publicationDate | 2018-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | BR-PI0713795-B1 |
| titleOfInvention | Method of optimizing a coding nucleotide sequence encoding a predetermined amino acid sequence |
| abstract | method for obtaining increased expression of polypeptides. The present invention relates to methods of optimizing sequences encoding a protein for expression in a certain host cell. the methods apply genetic algorithms to optimize single-codon aptitude and / or codon-pairing ability of sequences encoding a predetermined amino acid sequence. In the algorithm, the new sequence variant typing and subsequent selection of the most suitable variants are repeated until the variant coding sequences reach a minimum value for single codon suitability and / or codon pair suitability. The invention also relates to a computer comprising a memory processor, the processor being configured to read and write to memory, comprising data and instructions configured to provide the processor with the ability to perform genetic algorithms for optimizing condon fitness. single and / or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence of a predetermined amino acid sequence, the coding sequence being optimized for single codon suitability and / or codon pair suitability for a certain host in the methods of the invention. host cells comprising such nucleic acids and methods for producing polypeptides and other fermentation products in which such host cells are used. |
| priorityDate | 2006-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1031.