abstract |
METHOD FOR MANUFACTURING POLYPEPTIDEOS WITH ALDOLASE AND POLYNUCLEOTID ACTIVITIES THAT CODE THESE POLYPEPTIDS. This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and / or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of preparing and employing these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and / or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and preparing and employ these polynucleotides and polypeptides. The polypeptides according to the invention can be used in a variety of industrial, pharmaceutical and agricultural contexts. In some embodiments, the invention provides polypeptides and biosynthetic trails that are useful in the production of R-2-hydroxy 2- (indol-3-ylmethyl) -4-ketoglutaric acid (R-MP) and certain monatin stereoisomers, such as R, R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof. |