| abstract |
DOMAIN ANTIBODY CONSTRUCTION, NUCLEIC ACID ISOLATED MOLECULE, PHARMACEUTICAL COMPOSITION, AND METHODS TO DETECT HUMAN TNF-ALPHA IN A SAMPLE AND TO TREAT A DISTURBANCE The present invention provides a domain antibody construction that binds TNF- <24 > human, the construct comprising: (a) a domain antibody (dAb) that binds to human TNF-? (b) a modified hinge region sequence; (c) a human or primate heavy chain constant region sequence having a truncated C ~ H ~ 1 domain of no more than 20 residues, wherein said modified region of articulation sequence contains either a deletion or a single substitution of amino acid of at least one cysteine residue that normally facilitates disulfide bond formation between heavy and light chains of antibodies. |