| abstract |
Modified Xylanases That Show Enhanced Expression. A modified Family 11 xylanase enzyme comprising a sequence that introduces a functional consensus glycosylation site is provided. Non-limiting examples of introduced glycosylation sites include amino acid mutation at positions 34, 131, 180, 182, or a combination of these, for an asparagine. The indicated amino acid position in Family 11 xylanase is determined from the sequence alignment of the xylanase of interest with that of a Trichoderma reesei xylanase II amino acid sequence. The introduced consensus glycosylation site facilitates efficient expression of the modified xylanase compared to the efficiency of expression of a corresponding xylanase from which the modified xylanase was derived using host strains and similar growth conditions. |