abstract |
"recombinant carrier molecule for expression, release and purification of target polypeptides". recombinant carrier molecule that have thermostable enzyme amino acid sequences and methods of use for expression, recovery and release of foreign sequences (peptides and polypeptides) produced in different systems (bacteria, yeast, DNA, cell cultures as mammalian, plant, insect, protoplast and whole plant cells in vitro or in vivo) are provided. The recombinant carrier molecule that uses lichenase b (licb) sequences was also made and used as part of the carrier protein to express, retrieve, and release a variety of target polypeptides of interest. |