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filingDate 1997-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b959ecf2c734d02325f96e9a1b7d061f
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publicationDate 2000-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-9714675-A
titleOfInvention Rafinose synthase, process to produce raffinose, DNA, chimeric gene, plant, and process to change the oligosaccharide content of the raffinose family in a plant.
abstract "RAFINOSIS SYNTHASE, PROCESS TO PRODUCE RAFINOSIS, DNA, CHEMICAL GENE, PLANT, AND, PROCESS TO CHANGE THE CONTENT OF OLYMPOSACARIDES OF THE FAMILY OF RAFINOSIS IN A PLANT" Raffinose is produced by leaving raffinose synthase having the following properties to act on saccharose galactinol: (1) substrate action and specificity; produces raffinose from sucrose and galactinol, (2) optimum pH; about 6 to 8; (3) optimal temperature: about 35 to 40 ° C, (4) molecular weight: (i) about 75 kDa to 95 kDA evaluated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa evaluated by polyacrylamide gel electrophoresis (Native PAGE), and (iii) about 90 kDa to 100 kDa estimated by SDS polyacrylamide gel electrophoresis, under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and <MU> myo <MV> -inositol.
priorityDate 1997-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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