| abstract |
systems and methods for use and regeneration of chromatography. Aspects of the present description pertain to a method for regenerating a hydrophobic interaction chromatography column to which a mass of charge has been applied, the method comprising passing one or more column volumes of an alkaline solution through the hydrophobic interaction means within the column. , wherein the alkaline solution exhibits a pH between about 10 and about 14 and a conductivity between about 0.5 ms/cm and about 10 ms/cm, wherein material bound to the hydrophobic interaction media is removed. in some cases, the alkaline solution may include sodium hydroxide in a concentration between, for example, about 0.1 mm and 10 mm. |