| abstract |
methods for cell culture. A method for culturing cells is described, including (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite, selected from aconitic acid (aa), leucinic acid ( hica), cytidine monophosphate (cmp), methyl succinic acid (msa), trigonelline (tri) and n-acetylputrescinium (nap), below an inhibitory concentration in the cell culture medium, for at least one metabolite. |