| abstract |
Bisulfite-free whole genome methylation analysis. this disclosure provides methods for cost-effective bisulfite-free identification in DNA, including total genomic DNA, of the locations of one or more of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine, and 5-formylcytosine. the methods described in this document are based on the conversion of modified cytosine (5mc, 5hmc, 5fc, 5cac) to dihydrouracil (dhu), e.g., by tet-assisted pyridine borane treatment, followed by endonuclease cleavage from dhu and identification of the cleavage site, which corresponds to the location of the modified cytosine. |