| abstract |
methods for synthesizing anticoagulant polysaccharides. The present invention includes methods for preparing anticoagulant polysaccharides using various non-naturally occurring modified sulfotransferase enzymes that are designed to react with aryl sulfate compounds rather than the natural substrate, 3'-phosphoadenosine 5'-phosphosulfate, to facilitate transfer of sulfo group to sulfo group acceptor polysaccharides. suitable aryl sulfate compounds include, but are not limited to, p-nitrophenyl sulfate or 4-nitrocatechol sulfate. Anticoagulant polysaccharides produced by the methods of the present invention comprise n-, 3-o-, 6-o-sulfated glucosamine residues and 2-o-sulfated hexuronic acid residues, have comparable anticoagulant activity compared to commercially available anticoagulant polysaccharides, and can be used to form truncated anticoagulant polysaccharides having a reduced molecular weight. |