| abstract |
“MAMMALIAN CELLS, COMPOSITION, METHODS TO REDUCE OR ELIMINATE LACTOGENIC ACTIVITY IN A CELL, TO PRODUCE A PRODUCT OF INTEREST TO GROW A POPULATION”.nThe present disclosure relates to methods, cells and compositions for producing a product of interest, for example, a recombinant protein. In particular, the present disclosure provides enhanced mammalian cells that express the product of interest, wherein the cells (e.g., Chinese hamster ovary (CHO) cells) have modulated lactogenic activity. The present disclosure also relates to methods and compositions for modulating the expression of the pyruvate kinase (PKM) muscle (e.g., PKM-1 expression) in a mammalian cell to thereby reduce or eliminate the cell's lactogenic activity. , as well as compositions comprising a cell having reduced or eliminated lactogenic activity and methods of using these. |