| abstract |
The present invention relates to a method for separating a mature von Willebrand Factor (mat-VWF) from the von Willebrand Factor pro-peptide (VWF-PP) by incubating a composition comprising inducing dissociation of mat-VWF and VWF-PP by interruption of mat-VWF and VWF-PP not covalently associated, in which said dissociation is induced by: (i) adding at least one chelating agent, or (ii) increasing the pH to a pH of at least 7 and, in then, collection of said mat-VWF to obtain a mature VWF (mat-VWF) depleted of high purity pro-peptide. |