| abstract |
the invention provides a method for producing a hydrolyzed pectic polysaccharide isolate which is enriched in ramnogalacturonan-i, said method comprising the steps of: providing a substrate rich in pectin which was obtained from plant material without the use of organic solvent, said substrate rich in pectin containing at least 3% by weight of dry matter of pectic polysaccharides; subjecting the pectin-rich substrate to enzymatic treatment to partially hydrolyze pectic polysaccharides, said enzyme treatment comprising the use of one or more pectinases selected from pectin lyase (ec4.2.2.10), pectate lyase (ec 4.2.2.2) , ramnogalacturonan galacturonohydrolase (ec 3.2.1.173), endopoligalacturonase (ec 3.2.1.15), exopoligalacturonase (ec 3.2.1.67 and ec 3.2.1.82); subjecting partially hydrolyzed pectic polysaccharides to ultrafiltration using an ultrafiltration membrane that has a cut molecular weight in the range of 5 to 100 kda; and recover the ultrafiltration retentate. the present invention also relates to the hydrolyzed pectic polysaccharide isolate obtained by the present method and to a process for preparing a product selected from a nutritional formulation, a food product, a dietary supplement, a beverage or a pharmaceutical product, said process comprising adding the hydrolyzed pectic polysaccharide isolate mentioned above. |