http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-112020003339-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9288fab0395161486a1e9c3c2548a936 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6818 |
| filingDate | 2018-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_138bc363e060b526790d3b84c9b84cc5 |
| publicationDate | 2020-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | BR-112020003339-A2 |
| titleOfInvention | methods and kits for detecting nucleic acid molecules |
| abstract | it is a method for the detection of one or more polynucleotide amplification products, the method comprising, in one embodiment, the steps of: a) providing one or more oligonucleotide initiator groups, with each group comprising: i) a direct primer comprising a specific portion of fluorescence cassette and a specific portion of target 3 'downstream; and ii) one or more specific target primers; b) supply one or more extinct fluorescent pairs, each pair being characterized by: i) a first cassette oligonucleotide labeled with a chemical fluorescent portion and which has a sequence that is capable of hybridizing to the complement of the specific cassette portion of fluorescence of the direct primer of a given oligonucleotide primer group; and ii) a second cassette oligonucleotide labeled with a chemical extinction portion; c) initiate a polynucleotide isothermal amplification reaction in the presence of a strand-displacement DNA polymerase, generating a complementary sequence to at least a portion of the relevant direct primer, so that the second relevant cassette oligonucleotide is less able to hybridize with the first relevant oligonucleotide assay, by which a signal is generated; and d) detecting the signal. |
| priorityDate | 2017-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 267.