abstract |
methods and compositions of transducing / transfecting cells with a molecule, such as a nucleic acid (e.g., plasmid), are provided with high efficiency. high-efficiency transduced / transfected cells can, when transduced with a nucleic acid that encodes a protein or comprise a sequence that is transcribed into a transcription of interest, produce high amounts of protein and / or transcription. high efficiency transduced / transfected cells can, when transduced with plasmids comprising (i) nucleic acids encoding aav encapsulation proteins and / or nucleic acids encoding helper proteins and (ii) a transgene encoding a protein or being transcribed into a transcription of interest, produce large amounts of recombinant raav vector. |