| abstract |
the present invention relates to a method of preparing magnetic particles comprising a polymer matrix (p) and at least one magnetic core (m), preferably at least two magnetic cores (m), in which the polymer matrix ( p) comprises at least one hyper-crosslinked polymer and the method comprises (i) providing at least one magnetic core (m), preferably at least two magnetic cores (m); (ii) supply of polymer precursor molecules; (iii) polymerization of the polymer precursor molecules according to (ii) in the presence of at least one magnetic nucleus (m), so as to form a particle comprising at least one magnetic nucleus (m), preferably the at least two nuclei magnetic (m), embedded in a polymer matrix (p1); and (iv) hyper-reticulation of the polymer matrix (p1) of the polymer particle obtained in (iii) by means of a friedel-crafts reaction, in which the reaction is conducted under a temperature less than or equal to 80 ° c, to provide the magnetic particle. moreover, the present invention relates to particles obtained or that can be obtained by means of said method, as well as the use of those particles. in a further aspect, the present invention relates to a method of determining at least one substance to be analyzed in a fluid sample that comprises contacting the magnetic particle with a fluid sample that comprises or is suspected of comprising at least one analyte. |