| abstract |
Methods are provided for genetically modifying cells, including cells for use in connection with genetic engineering. in some embodiments, the methods provided including transducing cells by incubation with a retroviral vector particle, for example, lentiviral vector, wherein, prior to incubation, cells were not incubated with an activating or stimulating agent in the same manner as were not incubated with anti-cd3 / anti-cd28 antibodies and / or one or more recombinant cytokines. In some embodiments, such methods result in aspects related to shortening or improving the process for genetically modifying cells. The resulting cells transduced with a recombinant or heterologous gene, such as that encoding a chimeric receptor, such as a chimeric antigen receptor, or other recombinant antigen receptor such as a transgenic t-cell receptor, and compositions thereof are also provided. . In some embodiments, the cells and compositions provided may be used in adoptive immunotherapy methods. |