| abstract |
[problem] providing an improved ß-fructofuranosidase that is capable of efficiently producing cestosis while inhibiting the production of nystosis. [solution] this improved ß-fructofuranosidase comprises both: an amino acid sequence (a) obtained by introduction, in the amino acid sequence represented by sequence: 2, an amino acid mutation i) in which the 85th glycine (g) from the n-terminal is replaced by a protein-containing amino acid other than glycine (g) and / or an amino acid mutation ii) in which the 310th histidine (h) from the n-terminal is replaced by lysine (k), arginine (r ) or tyrosine (y); or an amino acid sequence (b) which exhibits ß-fructofuranosidase activity and which comprises an amino acid sequence obtained by deleting, replacing, inserting or adding one or more amino acids (a) other than the amino acid in which the amino acid mutation was introduced . |