abstract |
abstract expression of enzymes in yeast for lignocellulose-derived oligomer cbp The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood that can be expressed, for example, in yeast, such as saccharomyces cerevisiae. in some embodiments, this invention provides for the construction of a series of biocatalysts combining the expression and secretion of components of this enzyme system with the use of robust and rapid xylose and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. in some embodiments, the invention utilizes cocultures of strains that can achieve significantly improved performance, due to the incorporation of additional enzymes into the fermentation system. |