| abstract |
prongf mutant and method of preparing a biologically active human beta-ngf the present invention relates to a prongf mutant and the use thereof, in particular, the use of a human prongf mutant for the production of beta-ngf. the present invention describes a method of preparing a biologically active human beta-ngf from an inactive insoluble prongf mutant. a prongf mutant of the invention is replaced by any amino acid, but not arg or lys at the native protease cleavage site r¹sk³r4, at least at the positions r¹ and k³. corresponding to positions 101 and 103 of the human wild-type prongf sequence. |