| abstract |
biomass-derived acetate detoxification via metabolic conversion to ethanol, acetone, isopropanol or ethyl acetate. the present invention provides new metabolic pathways for detoxifying biomass-derived acetate via metabolic conversion to ethanol, acetone, isopropanol or ethyl acetate. more specifically, the invention provides a recombinant microorganism comprising one or more native and / or heterologous enzymes that function in one or more metabolic pathways constructed to obtain: (1) conversion of acetate to ethanol; (2) conversion of acetate to acetone; (3) conversion of acetate to isopropanol; or (4) conversion of acetate to ethyl acetate; in which one or more native and / or heterologous enzymes are activated, over-regulated or under-regulated. the invention also provides new organisms adapted to grow in the presence of inhibitory compounds, including, but not limited to acetate. |