| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14430ce0c28e36044927f5834e4d02ad |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-161
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-521 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate |
2012-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f9bddcb1707942d6a7c8160bb274009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9f4b5e10ae402877ecdbf5300e5e3d3 |
| publicationDate |
2021-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
BR-112012026221-B1 |
| titleOfInvention |
method and kit for detecting a target nucleic acid sequence of a dna or a mixture of nucleic acids using a ptoce assay |
| abstract |
method and kit for detecting target nucleic acid sequence of a dna or mixture of nucleic acids by means of a ptoce assay. the present invention relates to the detection of a target nucleic acid sequence by means of a ptoce assay (cleavage and extension with pto). the present invention detects a target nucleic acid sequence in which the pto (probing and labeling oligonucleotide) hybridizes to the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized to the cto (capture and template oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. the extended duplex provides signals (generation, increase, extinction or decrease of signals) starting from the markings that indicate the presence of the extended duplex and has an adjustable t m value, which are well adopted for detecting the presence of the target nucleic acid sequence. |
| priorityDate |
2011-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |