| abstract |
RNA preparations comprising purified modified RNA to reprogram cells. the present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-stranded mRNA encoding an ips cell induction factor. the purified RNA preparations are preferably substantially free of contaminating RNA molecules that: i) would activate an immune response in somatic cells, ii) decrease the expression of single-stranded mRNA in somatic cells, and / or iii) activate RNA sensors in somatic cells. in certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded mRNAs, uncapped RNA molecules and / or single-stranded mRNAs. |