http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-102013032960-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_220452bbf8b7868cd0719f7fd893a494 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate | 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10bfe23e0293dbfb4c6c92f645018cb0 |
| publicationDate | 2015-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | BR-102013032960-A2 |
| titleOfInvention | hdv quantifier method - hepatitis delta virus |
| abstract | hdv quantifier method - hepatitis delta virus'. The present invention relates to a HDV - hepatitis delta virus quantifier method, characterized by the use of reverse transcriptase followed by pcr, with simultaneous detection by fluorescence generated from the "5'-endonuclease" activity of taq dna polymerase on internally controlled hydrolysis - fam probes with endogenous hex control hydrolysis probes with quantification performed with rna hdv standard curve and analyzed by logarithmic regression or viral load versus cycle threshold - cv x ct, and where positive control and internal control were produced by a cloning system and the quantification of internal control by qpcr by measuring the hex fluorophore, with logarithmic dilutions ranging from 1 million to 1,000 copies / ml. A plasmid containing a modified hcv fragment was designed in line with a modified hdv fragment also and after in vitro production of stabilized rna from In the designed plasmid, the quantification of the internal control produced from the existing hcv quantitation panels was adjusted and extrapolated to the hdv internal control and the rna produced was purified, quantified and diluted. |
| priorityDate | 2013-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.