| abstract |
PROCESS FOR REACTION OF A CARBOXYLIC ACID ESTER, USE OF A KNOCKOUT OF A GENE ENCODING A POLYPEPTIDE, RECOMBINANT CELL AND ITS USE, AND REACTION MIXTURE The invention relates to a process for the reaction of a carboxylic acid ester of formula (1) R1-A-000R2 (1), wherein R1 is selected from the group, comprising hydrogen, -CH2OH, -CHO, -COOR3, -CH2SH, -CH2OR3, and -CH2NH2, wherein R2 is selected from the group , which comprises alkyl, preferably methyl, ethyl and propyl, wherein R3 is selected from the group comprising hydrogen and alkyl, preferably methyl, ethyl and propyl, and wherein in the case of A, it is an alkylene, alkenylene, arylene radical or substituted, unsubstituted, linear, branched and/or cyclic aralkylene of at least 4, more preferably 6, even more preferably 8 carbon atoms, via a cell, step a) comprising contacting the cell with the acid ester carboxylic acid in an aqueous solution, and in the case of the cell, if t rata of a recombinant cell, in which the activity of a polypeptide comprising SEQ ID NO 2 or a variant thereof relative to the wild type of the cell is reduced, as well as a cell suitable for that purpose and its uses. |