http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BR-102012004742-B1

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publicationDate 2021-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber BR-102012004742-B1
titleOfInvention METHOD AND KIT FOR SEROLOGICAL DIAGNOSIS OF CANINE VISCERAL LEISHMANIASIS BY FLOW CYTOMETRY TECHNIQUE
abstract KIT FOR SEROLOGICAL DIAGNOSIS OF CANINE VISCERAL LEISHMANIASIS, USING FIXED PROMASTIGOT FORMS BY THE FLOW CYTOMETRY TECHNIQUE. Diagnostic kit for the investigation of fixed IgG Anti-Promastigote Antibodies using flow cytometry. For that, there was the production of Leishmania infantum promastigotes that were preserved at different temperatures and preservatives. Quality control evaluations showed that formaldehyde at refrigerator temperature is the ideal condition for preserving antigens. The performance indices revealed excellent values for sensitivity, specificity, positive predictive value, negative predictive value and accuracy. Given the above, an alternative for the diagnosis of CVL becomes available, which can be used to control the disease in Brazil.
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