| abstract |
The human tumor necrosis factor (TNF) is used in medicine. TNFs and mutant proteins (muteins) have n-terminal deletions and higher biological activity. TNF is obtained by recombinant methods, the human promyelocytic leukemia cell line is induced by an improved method and TNF is purified to homogeneity. Methods, vectors and cells necessary for the preparation of human TNF in amounts sufficient for practical application are applied. 19 claims, 8 figures |