http://rdf.ncbi.nlm.nih.gov/pubchem/patent/BG-112835-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f05e0df5a62e4478dd64563d20dd86bc |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-716 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18 |
| filingDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b5ff343860c7c9e35819f766624941a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e009cbe1bc80dea7cdea37e09b9b61bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37e7099409d72746b44bdffb4a0d12fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e0c77dec96d3ea12f4b4c8a63ea1af8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a96bf3aac596068fbc96d2e342b46b60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a74f286a6d816485e79dcc8a0bb9d84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7572c407bf776925e1bc96090c80e4f0 |
| publicationDate | 2020-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | BG-112835-A |
| titleOfInvention | PREPARATION OF WATER-SOLUBLE GLUCAN BY TRANSFERASE ENZYME REACTION |
| abstract | The present patent application relates to the enzymatic synthesis of a water-insoluble glucan by a transferase reaction with a glucan-sucrase URE 13-300. The latter was obtained by culturing a recombinant strain of E. coli BL21 (DE3) containing the glucan sucrase URE 13-300 gene, which was transferred from the Leuconostoc mesenteroides strain URE 13 (registration number NBIMCC 8970, NBMPCC, Sofia). The water-insoluble glucan has a molecular weight of 2.49 x 106 Da and a radius of rotation (RG) of 60.0 to 2.78 x 106 and a radius of rotation of 67.0 and a branched structure, with a predominance of? - (1,6) linkages between glucose residues in the main chain (67.2%); 16.18%? - (1,3) connections at branch points and 8,32%? - (1,6) connections at branches. The high molecular weight glucan sucrase URE 13-300 is successfully immobilized in multilayer films with the participation of 1% chitosan and 0.2% xanthan / water-insoluble glucan, in a ratio of 1: 1 with the highest enzyme activity in a positively charged film with 12 layers and synthesizes short-chain oligosaccharides with a degree of polymerization of 4 to 6 glucose units. The synthesized glucan forms a film with antistatic properties in combination with locus bean and xanthan gum in a ratio of 1: 1: 1. |
| priorityDate | 2018-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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