| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_916075766043ce900399a4c2eee9f054 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25375
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-182
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-812
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-25125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-00 |
| filingDate |
1999-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2002-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da7ddd3b38cfd899480d10914905a3c0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10d0b4ca4b37e826d242ebf00e3c5d63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e910e53b1e2eb0a1c8fa53cccb6edab5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2074559c5c0e9f38a898d273dbb61981
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_616a86097af906e3fd6bca0b6488bf18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04731987ddd83aa1dfd5af8b579e76bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c8286b29dc6b9df81893a8d35863add |
| publicationDate |
2002-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
AU-755334-B2 |
| titleOfInvention |
Rapid quantitative analysis of proteins or protein function in complex mixtures |
| abstract |
Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amount of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or cell state of the cell, tissue or organism from which the sample originated. <IMAGE> |
| priorityDate |
1998-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |