http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2022252839-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d07a4ddba85ae3e35ca0a6f85dad9f6d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0655 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P43-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-32 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2022-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86ec7492e286da5d08bb9410aac17ec7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfb12d5fb8465aab86126de9af13194b |
| publicationDate | 2022-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2022252839-A1 |
| titleOfInvention | Methods and compositions for generating chondrocyte lineage cells and/or cartilage like tissue |
| abstract | Methods and compositions for generating chondrocyte lineage cells and/or cartilage nlike tissue nA method for generating chondrocytes and/or cartilage, optionally articular like non-hypertrophic nchondrocyte cells and/or cartilage like tissue and/or hypertrophic chondrocyte like cells and/or ncartilage like tissue, the method comprising: na. culturing a primitive streak-like mesoderm population, optionally a CD56+, nPDGFRalpha+ KDR- primitive streak-like mesoderm population, with a paraxial nmesoderm specifying cocktail comprising: ni. a FGF agonist; nii. a BMP inhibitor; optionally Noggin, LDN-193189, Dorsomorphin; and niii. optionally one or more of a TGFbeta inhibitor, optionally SB431524; and a nWnt inhibitor, optionally DKK1, IWP2, or XAV939; nto specify a paraxial mesoderm population expressing cell surface CD73, CD105 nand/or PDGFR-beta; nb. generating a chondrocyte precursor population comprising: ni. culturing the paraxial mesoderm population expressing CD73, CD105 and/or nPDGFR-beta at a high cell density optionally in serum free or serum ncontaining media; nii. culturing the high cell density CD73+, CD105+ and/or PDGFRbeta+ paraxial nmesoderm population with a TGFbeta3 agonist in serum free media to nproduce a high cell density Sox9+, collagen 2+ chondrocyte precursor npopulation; and nc. either ni. culturing the high cell density Sox9+, collagen 2+ chondrocyte precursor npopulation with the TGFbeta3 agonist for an extended period of time to nproduce an articular like non-hypertrophic chondrocyte cells and/or cartilage nlike tissue; or nii. culturing the high cell density Sox9+ collagen2+ chondrocyte precursor npopulation with a BMP4 agonist for an extended period of time to produce a nhypertrophic chondrocyte like cells and/or cartilage like tissue. |
| priorityDate | 2013-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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Total number of triples: 747.