| abstract |
Provided herein are cell surface conjugates containing a cell surface molecule and at nleast one agent, such as at least one affinity tag, and engineered cells expressing such cell nsurface conjugates. In some embodiments, the cell surface molecule does not contain an nintracellular signaling domain or is not capable of mediating intracellular signaling. In some nembodiments, the cells engineered to contain the cell surface conjugate, such as T cells, nfurther contain a genetically engineered recombinant receptor that specifically binds to nantigens, such as a chimeric antigen receptor (CAR). Also provided are methods of ndetecting, identifying, selecting or targeting cells expressing the cell surface conjugtes, such nas in connection with methods of manufacturing engineered cells or in connection with nadministration of such cells to subjects, including methods of adoptive cell therapy. |