| abstract |
The present disclosure discloses a paper-based chromatographic viral nucleic nacid extraction kit, and relates to the technical field of nucleic acid extraction. The npaper-based chromatographic viral nucleic acid extraction kit includes the following ncomponents: a lysis buffer, a cleaning solution, an eluent, and a chromatographic nextraction tube, wherein the chromatographic extraction tube is a 1.5 to 2.0 mL EP ntube additionally provided with a chromatography paper sheet and a chromatography ncotton. According to the paper-based chromatographic viral nucleic acid extraction kit, nformulations of the reagents are optimized based on a sample paper-based nchromatographic theory, trace amount of viral nucleic acid can be quickly captured nfrom 200 pL of sample, the operating process is very simple, the extraction and npurification of viral nucleic acid can be finished within 10 min, which saves a lot of ntime and reagents, and the extraction efficiency is significantly improved. Moreover, nno expensive instrument is required, compared with that of the existing methods, the ntest cost is significantly reduced, and thus it is suitable for Point of Care Testing n(POCT). The extraction method has the advantages of rapidness, high efficiency, nsimple operation, etc., and fully meets requirements of subsequent molecular ndetection such as PCR or RT-PCR, which saves a lot of time for testing, diagnosing nand research staffs. n- 10-nmpI.ncauon P0t n525000 n$00.000 n475,000 n450.000 n425-000 n400.000 n375.000 n350.000 n325 000 n300,000 n275000 n250000 n225000, The present n200 000 extraction method Control extraction n175000 method n150000 n125000 n100000 n75000 n50-000 n25000 n0 .99= n-25.000, nCycle nFig. 1 n- 11- |