| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b68f2d3b35fc944659350ec44702ca9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab6b26a5a3df910896fd0dc853568f8c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2fec0f568fea07c2ba5e95f2b2bb4604
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65563903b2b16d7e5d18ca064d5aa7d3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-112
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-13 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate |
2021-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2021-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c25e4250a6175fe968189ec62fe1d166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eb5e978ceaafafdb1e49ddd8c7c618c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_024d5757056b53ee5391c2d6e5ee375b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a0fbb3f08e27c6a3d0d4785c87b2d65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d73d54dd85f24bc38d8d9d97d3bd4302
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f248dc34d71f6eb549d6a4a96eb7fa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1da47a861e20b222d676eb4966a95692
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fca4086c4026439970d4cff7108cf82f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d8099e27b7f89c9228e9246eb1035a7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b27011e3efc781262670411c1acdec4b |
| publicationDate |
2021-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
AU-2021102785-A4 |
| titleOfInvention |
Primers, probe, kit and method for qpcr detection of phenacoccus manihoti |
| abstract |
The present invention provides primers, probe, kit and method for qPCR detection of Phenacoccus manihoti nwith high specificity and high sensitivity. The method for qPCR detection of Phenacoccus manihoti ncomprises: Step (1): extracting DNA of a sample to be detected; Step (2): preparing a reaction system; Step n(3): constructing recombinant plasmids; step (4): conducting qPCR amplification on the sample to be ndetected, plasmid sample, positive control sample and negative control sample using the primers and nprobe; Step (5): plotting standard curves; and Step (6): calculating a result.nurawings o uescripion nPM-Fl-+ nCTTTLACCTTTGATGATTTCTTCTTCTG TTTAATATTTCCACGAT nPM-Probel---+ nTAAATA TTTTAGATTTTGATTACTACTTCCTTCTTTAATATTATTA nATTTTAAATATAATATTAAACAATAATATTAATACAGGTTGGACTC n-PM-R1 nTTTACCCCCCATTAATTAAC'AAAATTTCATTACTTTAAACTTC nFig. 1 nStandard Curve n128 -n2 2 ....- . ... .......... ... ..-. .....- .....- .....-. ....-.. ......-. .. . n-5 -4 -3 -2 -1 0 nLog Starting Quantity nD Standard nX Unknown n-- FAM E=111-0% R^2=0.998 Slope=-3.OB4 y-int=12.14B nFig.2 n1 |
| priorityDate |
2021-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |