Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_850a17186ff51f03c607cf42a534a635 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dafbb8b19b5dd7177e0586bc6c224dcf |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-90 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0652 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0668 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P19-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
filingDate |
2020-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0002412145743ebbc9e838981fa9c0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1a11595056de8a09064340d27986248 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ee97123e283d547ffd9ecb4f8f52d51 |
publicationDate |
2021-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
AU-2020244911-A1 |
titleOfInvention |
Method of culturing cell population and use thereof |
abstract |
The present invention addresses the problem of providing a means for efficiently preparing a cell population, which contains abundant cells having a definite characteristic corresponding to the purpose of use (for example, type II collagen-positive nucleus pulposus cells), from a cell population containing Tie2-positive stem/progenitor cells (for example, nucleus pulposus stem/progenitor cells). The culture method according to the present invention comprises culturing a cell population containing Tie2-positive stem/progenitor cells while suppressing the formation of spherical colonies under the following conditions: (1) in a state of being present in a tissue which is not subjected to a digestion treatment; (2) in a medium which contains at least one Tie2 expression enhancer other than a growth factor; (3) in a culture container which is provided with a culture surface having been treated so as to enhance cell adhesion; or (4) in a medium which contains an extracellular matrix degrading agent. |
priorityDate |
2019-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |