| abstract |
OF THE DISCLOSURE nSome embodiments described herein relate to a substrate with a surface comprising a nsilane or a silane derivative covalently attached to optionally substituted cycloalkene or noptionally substituted heterocycloalkene for direct conjugation with a functionalized molecule nof interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a npolynucleotide, or a protein. In some embodiments, the silane or silane derivative contains noptionally substituted norbornene or norbomene derivatives. Method for preparing a nfunctionalized surface and the use in DNA sequencing and other diagnostic applications are also ndisclosed. |