| abstract |
The invention belongs to the field of chemical testing, and relates to a colorimetric nstrip, colorimetric card and test method for quickly testing histamine. The colorimetric nstrip is prepared by a method including the following steps: (1) preparing a PDA vesicle: nmixing and stirring 10,12-pentacosadiynoic acid and n1,2-dimyristoyl-sn-glycero-3-phosphocholine with an organic solvent, then evaporating nthe organic solvent, adding ultrapure water to dissolve a resulting mixture, then nperforming ultrasonic dispersion, and subsequently, storing a dispersion liquid at a low ntemperature in the dark; and then performing ultraviolet irradiation on the dispersion nliquid for polymerization to obtain a PDA vesicle dispersion liquid; and (2) assembling a ntesting colorimetric strip: fixing an adsorption carrier to a substrate, and dropping the PDA nvesicle dispersion liquid containing 0.2%-0.8% of trehalose as a chromogenic indication nliquid onto the adsorption carrier, to obtain the colorimetric strip. Compared with other ntesting methods, the colorimetric strip according to the invention is free of complex nbiochemical modification and instrumental testing, easy to store and carry and low in cost, nwith visible and simple test results.n3/3 n250 n225n- Y=14.2831og 2X+3.011 n200- R2 =0.9917 n175n150n125n100 1 1 1 n1024 4096 16384 65536 nHistamine Concentration (pM) nFIG. 3 |