| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0328dc1833863fe13dfa746048bec03 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2020-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ea58bdbe8de149c7c44404dcb84ac90
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d73314bf2e14c0927b3765828eec6633
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a79d20aa98967006feeeef814f2934e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b260bcaf720888d4e1e56486415fac5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f471909996713dd4bdb220f48e67642b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5087d7d85f1e448ee32ebe3d2d747b9 |
| publicationDate |
2020-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
AU-2020100740-A4 |
| titleOfInvention |
Base editor, preparation method and use thereof |
| abstract |
The present invention provides a base editor, a preparation method and use thereof, and belongs to the technical field of gene editing. The base editor includes a pCMV-dCpfl-RR-eBE recombinant plasmid and a pLbCpfl-sgRNA recombinant plasmid. The use includes the following steps: determining a target sequence and designing a single-stranded oligonucleotide pair; annealing to obtain a double-stranded DNA fragment; ligating to the pLbCpfl-sgRNA recombinant plasmid to obtain a targeting sgRNA expression vector; and culturing after cotransfection of cells with the targeting sgRNA expression vector and the pCMV-dCpfl-RR-eBE recombinant plasmid. The base editor of the present invention specifically mutates cytosine (C) to thymine (T) at a target site, but has no effect on bases at a non-target site. Gene editing efficiency ranges between 20% and 30%. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114250242-A |
| priorityDate |
2019-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |