http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2018202250-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_436c80ac97c18c9be85d896bfe12b8b5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-988 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9513 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-96472 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-948 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-17021 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-23016 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y402-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-23005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate | 2018-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b09497e39cf8385c9ea9813d2c73157 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4938d991de260c7ca63000aed61c6bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28ef729efae405151bea3f4774ab9017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a35fab4ce058398fecaa0a47e46965d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_623170bcb66f08bc908cfceab127c3bf |
| publicationDate | 2019-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2018202250-B2 |
| titleOfInvention | Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites |
| abstract | Abstract The invention provides a method for detection of active form of analytes in a sample and/or for determination of 5 ability of tested substances to bind to the active site of these analytes; comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent 10 binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively 15 binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample; b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding 20 to the analyte active site; whereas the probe consists of a low molecular compound for selective binding to the analyte active site; an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking 25 the compound for selective binding to the analyte active site and the oligonucleotide tag; c) then the solid carrier is washed to remove unbound detection probe; and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional 30 to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet 35 undetectable. 10076890_1 (GHMatters) P105105.AU.1 14/03/18 Incubation with Adsorption of antibody (Ab), sample containing surface blocking target enzyme (En) AbyO Ab Solidcarrier M Y cr 01 A 7 Incubation with the detection probe (inhibitor conjugated to DNA template) ssDNA Inhibitor 1) Wash 7 Ab 2) qPCR Solcarrier Fig.I A ssDNA B dsDNA C Biotin Inhibitor D E F Inhibitor Neu Neu Neu Neu Au inhibitor Fig.2 |
| priorityDate | 2014-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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