| abstract |
Disclosed herein are novel processes for the production of oligonucleotides that are suitable for use in the production of chemically modified oligonucleotides, such as those for use in therapy, said processes comprising: a) providing a template oligonucleotide (I) complimentary to the sequence of the product, said template having properties that allow it to be separated from the product; b) providing a pool of oligonucleotides (II); c) contacting (I) and (II) in conditions to allow annealing; d) changing the conditions to separate any impurities, comprising denaturing the annealed template and impurity oligonucleotide strands and separating the impurities; and e) changing the conditions to separate the product, comprising denaturing the annealed template and product oligonucleotide strands and separating the product. |