http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2017272256-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07d5f4667523063bce7464a42e3eb230 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2017-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7a5b4c1283b0f4f88fcb2fc34b5e14d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc651f5f04f820dfc4dfac18c36272da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bceddc04739d6b60dedfac2f5ce43377 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce832c0c9a5df814a530dfdb9fa3da36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5949ad27e5efaa821523879a8810c1ba |
| publicationDate | 2018-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2017272256-B1 |
| titleOfInvention | Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions |
| abstract | Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogen eous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains re arranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed f4 high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification. |
| priorityDate | 2012-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 165.