| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_08075d82e6c8945ac5f43e871640aca1 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91171
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2610-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2033-0096 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5085
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-551
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y205-01006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-551 |
| filingDate |
2016-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24bdf43ef0b4300c76de3240759e93e5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ef098999dffe4b5beebebd665e0a8c2d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91e02eef1929ccf8b72586c7b2ebbdf8 |
| publicationDate |
2020-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
AU-2016350141-B2 |
| titleOfInvention |
Methionine adenosyltransferase biological activity assay method and reagent kit |
| abstract |
A methionine adenosyltransferase (MAT) biological activity assay method and a reagent kit. A sample and a reagent are mixed according to certain proportions to cause a MAT-catalysed enzymatic reaction. The reaction and a competitive enzyme-linked immunosorbent assay assaying a reaction product S-adenosylmethionine (SAM) may be performed step-by-step or simultaneously. The MAT biological activity is calculated by calculating the amount of the product SAM produced per unit of time by assaying the absorbance of a tracer enzyme reaction product on a specific spectrum and comparing said absorbance with that of a standard product. A SAM quantification method is tagging a tracer to an anti-SAM antibody or SAM or a SAM analogue antigen, and causing produced SAM to compete with a certain amount of the SAM antigen for the anti-SAM antibody via the principle of competition. The method is used to assay and observe the MAT activity in normal and hepatoma cells and the effect of different regulatory factors on the MAT activity. |
| priorityDate |
2015-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |