| abstract |
Methods, systems, compositions, and thermochemical processes for inactivating proteinaceous binding entities (PBEs) such as antibodies in between staining cycles for preventing cross-reactivity in subsequent staining cycles. The thermochemical methods, compositions, or systems may feature incubating a sample in a solution comprising a buffer at a temperature sufficient to reduce or eliminate further detectability of the PBE. The methods, compositions, and systems may be beneficial for automated assays in automated staining devices that are adapted to dispense buffers and reagents and to heat samples to various temperatures. |