http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2016238891-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c37ea6c0ccf659261758e2450ac848e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate | 2016-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06a45d10b08c1b1e4a4928313d3071bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_583ceb82a5157d89e19bea093c326af9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c011f1c62ffa99d254422631af199a56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7b616bc859e7be88a5130ff562072e7 |
| publicationDate | 2016-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2016238891-A1 |
| titleOfInvention | Passaging and harvesting formulation and method for human pluripotent stem cells |
| abstract | Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyotype after passaged with the formulations for extended period of time. 90% 70% -- 0% - -iEDTA -I EGTA . 40% --- - ENa t 20% - 1 0% - 0 1 N-a 0.1 0.55 1 3 10 1X Concentration (mM) Sodium citrate disrupts cell-surface bond more than EDTAIEGTA. hiESC colonies treated with sOdium citrate do not re-stick onto the culturing surface after lnin incubation in MEF-CM. |
| priorityDate | 2011-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 77.