http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2015270774-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_671039dce45aa9f90e0ccc011e8fe257 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c842dc5f6c6303d1b4064e28d4247496 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_135f30b72e489f20d4003baa11862aca |
| publicationDate | 2021-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | AU-2015270774-B2 |
| titleOfInvention | Optimization of DNA amplification reactions |
| abstract | The present invention relates to methods and uses where a combination of (i) an enzyme exhibiting 5'->3' exonuclease activity and specifically degrading 5'-pohsphorylated strands of double-stranded nucleic acids is used, and (ii) one or more primer pair(s), each primer being 5' phosphorylated, in a method comprising at least a first and a second amplification reaction of a first and a second template, respectively, for preventing contamination of the second amplification reaction with amplification products of the first amplification reaction, as well as novel methods and kits using the combination. Also, the present invention relates to kits comprising the enzyme and either 5'-phosphorylated primers or a polynucleotide kinase. |
| priorityDate | 2014-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 116.