Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f9be9b367fe2af9a048c8620487d4f9 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-525 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-101 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate |
2015-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2020-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3aaa23d4958c426563585cfd03e4001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5147b70dcac5f77af6821b85782d0f9 |
publicationDate |
2020-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
AU-2015210705-B2 |
titleOfInvention |
Improved methods for processing DNA substrates |
abstract |
The present disclosure describes a method of adapter ligation to the ends of fragmented double-stranded DNA molecules. This novel method overcomes the necessity to add a phosphate group to the 5' ends of DNA fragments. Instead, the 5' terminal bases that are damaged as a result of physical fragmentation of the DNA, are removed. By removal of the damaged base, a ligation compatible base with a 5' phosphate is exposed and adapter ligation efficiency is restored, leading to a significant increase in library yield and the ability to construct libraries from reduced input DNA quantities. |
priorityDate |
2014-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |